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. Author manuscript; available in PMC: 2022 May 6.
Published in final edited form as: Mol Cell. 2021 Mar 15;81(9):1970–1987.e9. doi: 10.1016/j.molcel.2021.02.031

Fig.7: Selective cohesin eviction and retention are required to maintain Polycomb targets, fold the Xi, and properly silence Xi genes.

Fig.7:

(A) Cumulative distribution plots of chromosome-wide Xi expression in 2 WAPL-FKBPdegron clones treated from days 4–7 of differentiation with DMSO or 500nM dTAG-13. P-value calculated by the KS test.

(B) Cumulative distribution plots of Xi expression from the centromeric most 30Mb, middle 100Mb, and telomeric most 30Mb of the chromosome in 2 in 2 WAPL-FKBPdegron clones treated as in (A). P-value calculated by the KS test.

(C) Examples of genes at the far centromeric (Clcn5,Tspan7) and telomeric (Apex2,Ap1s2) ends of the X chromosome. Allelic RNA-seq and H3K27me3 cChIP-seq shown for 2 WAPL-FKBPdegron clones treated as in (A).

(D) Model: cohesin tempering of Polycomb targets on autosomes.

(E) Model: cohesin tempering of Xist and Polycomb spreading on the Xi.