| Antibodies |
| Monoclonal mouse anti-StrepII |
Qiagen |
cat#34850 |
| Monoclonal mouse anti-His |
Genescript |
cat#A00186 |
| Polyclonal rabbit anti-EspP β-barrel (N-term peptide) |
Doyle and Bernstein, 2019 |
N/A |
| Polyclonal rabbit anti-EspP β-barrel (C-term peptide) |
Szabady et al., 2005 |
N/A |
| Polyclonal rabbit anti-BamA (C-term peptide) |
Pavlova et al., 2013 |
N/A |
| Polyclonal rabbit anti-BamA (full protein) |
Ieva and Bernstein, 2009 |
N/A |
| Goat anti-mouse Ig 800CW IRDye |
Licor |
cat#926-32210 |
| Goat anti-rabbit Ig 680LT IRDye |
Licor |
cat#926-680210 |
| Bacterial and Virus Strains |
|
E. coli B strain BL21(DE3) |
Invitrogen |
cat#C600003 |
|
E. coli K-12 strain AD202 |
Akiyama and Ito, 1990 |
N/A |
|
E. coli K-12 strain RI2 |
Ieva et al., 2008 |
N/A |
|
E. coli K-12 strain XL1-Blue |
Agilent |
cat#200236 |
| Chemicals, Peptides, and Recombinant Proteins |
| 4,4’-dipyridyl disulfide |
Sigmaaldrich |
cat#143057-5G |
| Proteinase K |
Roche |
cat#3115852001 |
| BugBuster Master Mix |
EMD Milipore |
cat#71456 |
| Deposited Data |
| Uncut immunoblots |
This paper; Mendeley Data |
http://dx.doi.org/10.17632/3cy3y7dnmk.1 |
| Experimental Models: Organisms/Strains |
|
E. coli B strain BL21(DE3) |
Invitrogen |
cat#C600003 |
|
E. coli K-12 strain AD202 |
Akiyama and Ito, 1990 |
N/A |
|
E. coli K-12 strain RI2 |
Ieva et al., 2008 |
N/A |
| Oligonucleotides |
| See Table S8 in Supplementary Information
|
This paper |
N/A |
| Recombinant DNA |
| See Table S7 in Supplementary Information
|
This paper |
N/A |
| Software and Algorithms |
| UCSF Chimera |
Pettersen et al., 2004 |
https://www.cgl.ucsf.edu/chimera/ |
| Other |
| SMART website |
Letunic and Bork, 2018 |
http://smart.embl-heidelberg.de |
| Skylign webtool |
Wheeler et al., 2014 |
http://skylign.org |
