Scatterplot showing clustering of WT and mutant Tm values from samples tested in the clinical study. One hundred M. tuberculosis-positive frozen sputum samples and 214 clinical isolates for all Xpert MTB/XDR targets were tested and plotted using ggplot in R studio. To prevent overplotting, a degree of jitter was introduced. All green dots are WT Tm, while brown, purple, blue, and pink are mutant Tm values.