TABLE 1.
Tm values for gyrA probes and resistance detection
| Genotypea | Tm category | Probe Tm (°C) (±SD), Δ, or window type for associated phenotypeb |
FLQ resistance output | ||
|---|---|---|---|---|---|
| Probe 1 | Probe 2 | Probe 3 | |||
| WT | Mean | 76.2 (±0.2) | 70 (±0.2) | 70.8 (±0.2) | Not detected |
| Window | WT | WT | WT | ||
| G88C | Mean | 72.7 (±0.3) | 65.2 (±0.4) | 66.4 (±0.3) | Detected |
| Δ | −3.4 | −4.8 | −4.4 | ||
| Window | Mut B | Mut B | Mut C | ||
| G88A | Mean | 71.7 (±0.5) | 63.9 (±0.3) | 65.4 (±0.3) | Detected |
| Δ | −4.4 | −6.1 | −5.4 | ||
| Window | Mut B | Mut B | Mut C | ||
| A90V | Mean | 72.2 (±0.2) | 75.6 (±0.3) | 76.2 (±0.2) | Low resistance detected |
| Δ | −4.1 | 5.6 | 5.4 | ||
| Window | Mut B | Mut A | Mut B | ||
| S91P | Mean | 72.2 (±0.1) | 74.8 (±0.1) | 66.1 (±0.4) | Low resistance detected |
| Δ | −3.9 | 4.8 | −4.7 | ||
| Window | Mut B | Mut A | Mut C | ||
| D94A | Mean | 78.9 (±0.2) | 73.4 (±0.2) | 71.4 (±0.1) | Low resistance detected |
| Δ | 2.7 | 3.4 | 0.6 | ||
| Window | Mut A | Mut A | WT | ||
| D94G | Mean | 76 (±0.2) | 69.5 (±0.3) | 75.8 (±0.2) | Detected |
| Δ | −0.1 | −0.5 | 5 | ||
| Window | WT | WT | Mut B | ||
| D94N | Mean | 72.9 (±0.3) | 66.1 (±0.4) | 68.9 (±0.3) | Detected |
| Δ | −3.2 | −3.9 | −1.9 | ||
| Window | Mut B | Mut B | WT | ||
| D94Y | Mean | 72.5 (±0.3) | 65.1 (±0.4) | 68.6 (±0.3) | Detected |
| Δ | −3.6 | −4.9 | −2.2 | ||
| Window | Mut B | Mut B | Mut C | ||
| D94H | Mean | 73.2 (±0.3) | 65.6 (±0.3) | 68.9 (±0.3) | Detected |
| Δ | −2.9 | −4.4 | −1.9 | ||
| Window | WT | Mut B | WT | ||
| A90V+S91P | Mean | 67.5 (±0.3) | 79.3 (±0.2) | 71.7 (±0.1) | Detected |
| Δ | −8.6 | 9.3 | 0.9 | ||
| Window | Mut C | Mut A | WT | ||
| A90V+G88C | Mean | 67.8 (±0.5) | 71.3 (±0.2) | 72.2 (±0.2) | Detected |
| Δ | −8.3 | 1.3 | 1.4 | ||
| Window | Mut C | WT | WT | ||
a
All include 95S or T.
b
Tm values are means and standard deviations (SDs) from 3 to 163 replicates from multiple experiments depending on genotype for the three gyrA probes with representative gyrA mutant plasmids and their corresponding windows. WT windows are indicated, and the three mutant windows, MutA, MutB, and MutC, are light gray, dark gray, and dark gray with boldface, respectively.