TABLE 5.

PCR/sequencing of positive Unyvero specimens negative by SoC testing

Species	Study arm	No. of false-positive specimens	No. confirmed by PCR followed by sequencing/no. of false-positive specimens	No. with possible cross-reactivity/total (%)
Acinetobacter species	Prospective	11	11/11
	Archived	3	3/3
	Total	14	14/14 (100.0)
C. freundii	Prospective	3	3/3
	Archived	5	4/5	1/5
	Total	8	7/8 (87.5)	1/8 (12.5)a
E. cloacae complex	Prospective	7	7/7
	Archived	0
	Total	7	7/7 (100.0)
E. coli	Prospective	30	18/30
	Archived	17	15/17
	Total	47	33/47 (70.2)
H. influenzae	Prospective	48	42/48	5/48
	Archived	21	17/21	4/21
	Total	69	59/69 (85.5)	9/69 (13.0)b
K. oxytoca	Prospective	8	7/8
	Archived	6	6/6
	Total	14	13/14 (92.9)
K. pneumoniae	Prospective	10	5/10
	Archived	10	7/10
	Total	20	12/20 (60.0)
K. variicola	Prospective	2	2/2
	Archived	4	2/4
	Total	6	4/6 (66.7)
M. catarrhalis	Prospective	13	13/13
	Archived	9	8/9
	Total	22	21/22 (95.5)
M. morganii	Prospective	3	3/3
	Archived	0
	Total	3	3/3 (100.0)
Proteus species	Prospective	6	6/6
	Archived	7	6/7
	Total	13	12/13 (92.3)
P. aeruginosa	Prospective	43	41/43
	Archived	2	2/2
	Total	45	43/45 (95.6)
S. marcescens	Prospective	5	4/5
	Archived	3	1/3
	Total	8	5/8 (62.5)
S. aureus	Prospective	41	31/41
	Archived	21	18/21
	Total	62	49/62 (79.0)
S. maltophilia	Prospective	22	21/22
	Archived	10	10/10
	Total	32	31/32 (96.9)
S. pneumoniae	Prospective	10	10/10
	Archived	5	4/5
	Total	15	14/15 (93.3)

^aIn one of eight false-positive C. freundii cases, PCR/sequencing identified Citrobacter youngae.

^bIn 9 of 69 false-positive H. influenzae cases, PCR/sequencing identified Haemophilus haemolyticus (5 cases), Aggregatibacter aphrophilus (3 cases), or Haemophilus parainfluenzae (1 case).