TABLE 8.
Genotypic agreement and phenotypic agreement of Unyvero resistance marker results with isolates for corresponding specimens, with 95% confidence intervals
| Type of resistance | Genotypic agreementa | Phenotypic agreement (PPV)b | ||
|---|---|---|---|---|
| Resistance to 3rd-generation cephalosporins in Enterobacterales, P. aeruginosa, or Acinetobacter species | Unyvero results | No. of isolates harboring blaCTX-M/total, % (95% CI) | No. of isolates not harboring blaCTX-M/total, % (95% CI) | No. of blaCTX-M genes detected and resistant phenotype of isolate(s)/total, % (95% CI) |
| Host(s) and blaCTX-M | 3/3, 100.0 (43.9–100.0) | 4 | 12/12, 100.0 (75.8–100.0) | |
| Host(s) without blaCTX-M | 0 | 117/124, 94.4 (88.8–97.2) | ||
| Host(s) not detected | 0 | 3 | ||
| Resistance to carbapenems in Enterobacterales, P. aeruginosa, or Acinetobacter species | Unyvero results | No. of isolates harboring resistance marker(s)/total, % (95% CI) | No. of isolates not harboring resistance marker(s)/total, % (95% CI) | No. of resistance markers detected and resistant phenotype of isolate(s)/total, % (95% CI) |
| Host(s) and marker(s) | 2/2,c 100.0 (34.2–100.0) | 3 | 2/2, 100.0 (34.2–100.0) | |
| Host(s) without marker(s) | 0 | 119/125, 95.2 (89.9–97.8) | ||
| Host(s) not detected | 0 | 3 | ||
| Resistance to carbapenems conferred by the blaOXA panel markers in Acinetobacter species | Unyvero results | No. of isolates harboring resistance marker(s)/total, % (95% CI) | No. of isolates not harboring resistance marker(s)/total, % (95% CI) | No. of resistance markers detected and resistant phenotype of isolate/total, % (95% CI) |
| Acinetobacter species and blaOXA | 6/6,d 100.0 (61.0–100.0) | 0 | 8/9, 88.9 (56.5–98.0) | |
| Acinetobacter species without blaOXA | 0 | 5/5, 100.0 (56.6–100.0) | ||
| Acinetobacter species not detected | 0 | 0 | ||
| Resistance to penicillin in H. influenzae | Unyvero results | No. of H. influenzae isolates harboring blaTEM/total, % (95% CI) | No. of H. influenzae isolates not harboring blaTEM/total, % (95% CI) | No. of blaTEM genes detected and resistant phenotype of the isolate/total, % (95% CI) |
| H. influenzae and blaTEM | 4/4, 100.0 (51.0–100.0) | 0 | 17/19, 89.5 (68.6–97.1) | |
| H. influenzae without blaTEM | 0 | 6/6, 100.0 (61.0–100.0) | ||
| H. influenzae not detected | 0 | 0 | ||
| Resistance to oxacillin (methicillin) in S. aureus | Unyvero results | No. of S. aureus isolates harboring mecA/total, % (95% CI) | No. of S. aureus isolates not harboring mecA/total, % (95% CI) | No. of mecA genes detected and resistant phenotype of the isolate/total, % (95% CI) |
| S. aureus and mecA | 21/26, 80.8 (62.1 to 91.5) | 4 | 47/59, 79.7 (67.7–88.0) | |
| S. aureus without mecA | 2 | 24/30, 80.0 (62.7–90.5) | ||
| S. aureus not detected | 3 | 2 | ||
Genotypic agreement was determined for the subset of positive specimens for which isolates from corresponding host organisms were available for whole-genome sequencing.
Phenotypic agreement of positive resistance markers (positive predictive value) was determined for the subset of SoC-positive specimens with available antimicrobial susceptibility test results for the corresponding host organisms. For a comparison of all samples with resistance markers reported by Unyvero to SoC results, please refer to Table S5 in the supplemental material.
Two isolates distributed to the following host/marker combinations: E. cloacae complex/blaKPC and K. pneumoniae/blaNDM blaOXA-48.
Six Acinetobacter isolates harbored the following blaOXA genes: blaOXA-23 (3 cases) and blaOXA-24 (3 cases).