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. 2021 Feb 18;59(3):e02880-20. doi: 10.1128/JCM.02880-20

TABLE 2.

COVID-19 Ag card rapid test across a range of SARS-CoV-2-positive specimensa

UW sample ID Matrix medium Panther Fusion CT Original no. of copies/ml No. of copies/swab No. of copies/ml after dilution in 3 ml Theoretical Panther Fusion CT BinaxNOW antigen detection
8 VTM 14.6 3.29 × 108 1.65 × 107 5.49 × 106 21.0 DET
3 PBS 17.1 6.65 × 107 3.33 × 106 1.11 × 106 23.5 DET
13 VTM 17.1 6.65 × 107 3.33 × 106 1.11 × 106 23.5 DET
9 VTM 17.4 5.49 × 107 2.75 × 106 9.15 × 105 23.8 DET
72 VTM 17.6 4.83 × 107 2.42 × 106 8.06 × 105 24.0 DET
67 VTM 17.8 4.25 × 107 2.13 × 106 7.09 × 105 24.2 DET
19 VTM 18 3.74 × 107 1.87 × 106 6.24 × 105 24.4 DET
54 VTM 18 3.74 × 107 1.87 × 106 6.24 × 105 24.4 DET
71 VTM 18.1 3.51 × 107 1.76 × 106 5.85 × 105 24.5 DET
60 VTM 18.2 3.29 × 107 1.65 × 106 5.49 × 105 24.6 DET
16 VTM 18.5 2.72 × 107 1.36 × 106 4.53 × 105 24.9 DET
44 VTM 18.7 2.39 × 107 1.20 × 106 3.99 × 105 25.1 DET
42 VTM 19.3 1.63 × 107 8.15 × 105 2.72 × 105 25.7 DET
47 VTM 19.5 1.43 × 107 7.17 × 105 2.39 × 105 25.9 DET
64 VTM 19.7 1.26 × 107 6.31 × 105 2.10 × 105 26.1 DET
6 PBS 19.9 1.11 × 107 5.55 × 105 1.85 × 105 26.3 DET
10 VTM 19.9 1.11 × 107 5.55 × 105 1.85 × 105 26.3 DET
69 VTM 21 5.50 × 106 2.75 × 105 9.16 × 104 27.4 DET
17 VTM 21.2 4.84 × 106 2.42 × 105 8.06 × 104 27.6 DET
63 VTM 21.2 4.84 × 106 2.42 × 105 8.06 × 104 27.6 DET
48 VTM 21.5 3.99 × 106 2.00 × 105 6.65 × 104 27.9 DET
61 VTM 22 2.90 × 106 1.45 × 105 4.83 × 104 28.4 DET
12 VTM 22.1 2.72 × 106 1.36 × 105 4.53 × 104 28.5 DET
52 VTM 22.1 2.72 × 106 1.36 × 105 4.53 × 104 28.5 DET
70 VTM 22.5 2.11 × 106 1.05 × 105 3.51 × 104 28.9 DET
22 VTM 23.1 1.44 × 106 7.18 × 104 2.39 × 104 29.5 DET
51 VTM 23.4 1.18 × 106 5.92 × 104 1.97 × 104 29.8 DET
49 VTM 23.8 9.17 × 105 4.59 × 104 1.53 × 104 30.2 NOT DET
58 VTM 23.9 8.60 × 105 4.30 × 104 1.43 × 104 30.3 NOT DET
65 VTM 24.2 7.10 × 105 3.55 × 104 1.18 × 104 30.6 DET
57 VTM 24.4 6.25 × 105 3.12 × 104 1.04 × 104 30.8 DET
32 VTM 24.7 5.16 × 105 2.58 × 104 8.60 × 103 31.1 NOT DET
23 VTM 24.8 4.84 × 105 2.42 × 104 8.06 × 103 31.2 NOT DET
1 PBS 25 4.26 × 105 2.13 × 104 7.10 × 103 31.4 DET
68 VTM 25.1 3.99 × 105 2.00 × 104 6.66 × 103 31.5 NOT DET
43 VTM 25.4 3.30 × 105 1.65 × 104 5.50 × 103 31.8 NOT DET
59 VTM 25.4 3.30 × 105 1.65 × 104 5.50 × 103 31.8 DET
29 VTM 26.9 1.26 × 105 6.32 × 103 2.11 × 103 33.3 NOT DET
55 VTM 27.1 1.11 × 105 5.56 × 103 1.85 × 103 33.5 DET
27 VTM 27.2 1.04 × 105 5.21 × 103 1.74 × 103 33.6 DET
33 VTM 27.4 9.18 × 104 4.59 × 103 1.53 × 103 33.8 NOT DET
35 VTM 27.7 7.58 × 104 3.79 × 103 1.26 × 103 34.1 DET
38 VTM 27.7 7.58 × 104 3.79 × 103 1.26 × 103 34.1 DET
21 VTM 28.1 5.87 × 104 2.93 × 103 9.78 × 102 34.5 NOT DET
37 VTM 28.1 5.87 × 104 2.93 × 103 9.78 × 102 34.5 DET
62 VTM 28.1 5.87 × 104 2.93 × 103 9.78 × 102 34.5 NOT DET
53 VTM 28.3 5.16 × 104 2.58 × 103 8.60 × 102 34.7 NOT DET
2 PBS 28.4 4.84 × 104 2.42 × 103 8.07 × 102 34.8 DET
46 VTM 28.4 4.84 × 104 2.42 × 103 8.07 × 102 34.8 NOT DET
45 VTM 28.6 4.26 × 104 2.13 × 103 7.10 × 102 35.0 NOT DET
11 VTM 29 3.30 × 104 1.65 × 103 5.50 × 102 35.4 NOT DET
26 VTM 29 3.30 × 104 1.65 × 103 5.50 × 102 35.4 NOT DET
41 VTM 29 3.30 × 104 1.65 × 103 5.50 × 102 35.4 NOT DET
34 VTM 29.1 3.09 × 104 1.55 × 103 5.16 × 102 35.5 NOT DET
66 VTM 29.4 2.55 × 104 1.28 × 103 4.26 × 102 35.8 NOT DET
56 VTM 30.1 1.63 × 104 8.16 × 102 2.72 × 102 36.5 NOT DET
18 VTM 30.6 1.19 × 104 5.93 × 102 1.98 × 102 37.0 NOT DET
50 VTM 30.8 1.04 × 104 5.22 × 102 1.74 × 102 37.2 NOT DET
39 VTM 31 9.18 × 103 4.59 × 102 1.53 × 102 37.4 NOT DET
36 VTM 31.3 7.58 × 103 3.79 × 102 1.26 × 102 37.7 NOT DET
25 VTM 31.6 6.26 × 103 3.13 × 102 1.04 × 102 38.0 NOT DET
14 VTM 31.7 5.87 × 103 2.93 × 102 9.78 × 101 38.1 NOT DET
24 VTM 32.1 4.54 × 103 2.27 × 102 7.57 × 101 38.5 NOT DET
30 VTM 34.1 1.26 × 103 6.32 × 101 2.11 × 101 40.5 NOT DET
20 VTM 35 7.11 × 102 3.56 × 101 1.19 × 101 >40.5 NOT DET
31 VTM 35.1 6.67 × 102 3.34 × 101 1.11 × 101 >40.5 NOT DET
4 PBS 36.2 3.30 × 102 1.65 × 101 5.50 >40.5 NOT DET
28 VTM 36.4 2.91 × 102 1.45 × 101 4.84 >40.5 NOT DET
40 VTM 36.8 2.25 × 102 1.13 × 101 3.75 >40.5 NOT DET
15 VTM 38.1 9.80 × 101 4.9 1.63 >40.5 NOT DET
5 PBS 38.8 6.26 × 101 3.13 1.04 >40.5 NOT DET
7 PBS 39.9 3.10 × 101 1.55 0.52 >40.5 NOT DET
a

Abbreviations: Ag, antigen; UW, University of Washington; CT, cycle threshold; VTM, viral transport medium; PBS, phosphate-buffered saline; DET, detected; NOT DET, not detected. The conversion factor for copies per swab is 1/20 of copies per milliliter. Theoretical Panther Fusion CTs were calculated with RNA standards factoring in a 1:20 dilution for the copies per swab and then an additional dilution in 3 ml of transport medium. Hologic Panther Fusion CTs of >40.5 are expected to be missed.