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. 2021 Apr 22;19(4):321–332. doi: 10.18502/ijrm.v19i4.9058

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Copyright © 2021 Majidi Gharenaz et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. which permits unrestricted use and redistribution provided that the original author and source are credited.

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Characterization of testicular scaffolds. Macroscopic images showed that scaffolds were completely translucent (A) while native testes were opaque (B). Histological comparison of scaffolds (C) and native testes (D) by H&E staining exhibited the elimination of the cells. Original magnification 100x. DNA quantification confirmed the removal of 98% of the DNA from the tissue (E). a indicated significant difference with native testis (p $<$ 0.05). Masson's trichrome staining showed collagen preservation in scaffolds (F) and native testes (G). Alcian blue staining confirmed GAGs retention in scaffolds (H) and native testes (I). Original magnification 100 $\times$ .