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Sequence of primers and probes used for digital polymerase chain reaction (ddPCR)
| Primer and probes | Sequence (5′-3′) |
|---|---|
| PIK3CA-F | GCTCAAAGCAATTTCTACACGA |
| PIK3CA-R | CTTACCTGTGACTCCATAGAAAATC |
| PIK3CA-P-G | 6-FAM- TGAAATCACTGAGCAGGA- BHQ-X |
| PIK3CA-P-A | HEX- TGAAATCACTAAGCAGG- BHQ-X |
| PIK3CD-F | TCCGAGATGCACGTGCC |
| PIK3CD-R | CCTTCATGTGGTGGGTGCT |
| PIK3CD-P–T | 6-FAM -TTCGGCCTCATCCT- BHQ-X |
| PIK3CD-P–C | HEX -TTCGGCCCCATCC- BHQ-X |
