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Sequence of primers used for quantitative real-time PCR (RT-qPCR)
| Primer | Sequence (5′-3′) |
|---|---|
| GAPDH-F | 5ʹ- GGAGCGAGATCCCTCCAAAAT -3ʹ |
| GAPDH-R | 5ʹ- GGCTGTTGT CATACTTCTCATGG -3ʹ |
| mTOR-F | 5ʹ- ATGCTTGGAACCGGACCTG -3ʹ |
| mTOR-R | 5ʹ-TCTTGACTCATCT CTCGGAGTT -3ʹ (reverse) |
| AKT(Human)-F | 5′-CTACCCACACAGCAGTACGC-3' |
| AKT(Human)-R | 5′-AAGTCGCTGGTGTTAAGCCG-3' |
| S6-F | 5ʹ- AGGGTTATGTGGTCCGAATCA -3ʹ |
| S6-R | 5ʹ- TTGGTCTGT AACAGGAATGCC -3ʹ |
