Fig. 5.

Loss of EMP3 in GBM cells promotes T cell infiltration via CXCL10 and CXCL9 in macrophages. a-b CXCL9 and CXCL10 mRNA and protein expression in RAW264.7 cells exposed to supernatants from EMP3_KO or EMP3_Scra GL261 cells for 48 hours was detected by qRT-PCR and ELISA. Student’s t-test was performed. KO: Knockout; Scra: Scramble. c Chemotaxis of T cells towards RAW264.7 cells treated with IFN-γ, anti-CXCL9 and/or CXCL10. Student’s t-test was performed.d Bioluminescent images of C57BL/6 mice treated with an anti-αCXCR3 antibody in the EMP3_KO and EMP3_Scra groups on days 14, 21 and 35. KO: Knockout; Scra: Scramble. e Quantification of the bioluminescence imaging signal intensities in C57BL/6 mice. Student’s t-test was performed. f Kaplan-Meier survival curves of mice bearing intracranial EMP3_KO or EMP3_Scra GL261 tumours treated with the anti-CXCR3 antibody or isotype IgG. KO: Knockout; Scra: Scramble. The log-rank test was performed. g-h Percentages of TNFα⁺ CD4⁺and TNFα⁺ CD8⁺ T cells in tumours from the EMP3_KO and EMP3_Scra GL261 groups treated with the anti-CXCR3 antibody or isotype IgG. Student’s t-test was performed. KO: Knockout; Scra: Scramble. i-j Percentages of IFN-γ⁺ CD4⁺ and IFN-γ⁺ CD8⁺T cells in tumours from the EMP3_KO and EMP3_Scra GL261 groups treated with the anti-CXCR3 antibody or isotype IgG. Student’s t-test was performed. KO: Knockout; Scra: Scramble.k Pearson correlation plots of the expression of EMP3 and CXCR3 in 470 GBM cases and the definition of 3 subgroups according to the expression patterns. l Kaplan-Meier survival analysis according to the EMP3-CXCR3 subgroups, as defined in (k). The log-rank test was performed. The mean ± S.D. is shown. Ns: nonsignificant, *p < 0.05, **p < 0.01, and ***p < 0.001