ACE2 expression in a CD4+ T cell subset increases after ex vivo stimulation
(A) Comparison of immune features derived from each leukocyte subpopulation between experimental groups. A dot plot displaying the ES calculated in HAP versus COVID-19s (x axis) compared with the ES calculated in COVID-19m versus COVID-19s (y axis). Each dot represents one immunological feature. The red box highlights the immune feature focused in this figure.
(B) Median expression of indicated markers in FlowSOM-derived clusters of unstimulated samples.
(C) Median frequency and 25th and 75th percentiles of ACE2-positive cells in a subset of unstimulated CXCR3+ CCR6+ (Th1 Th17-enriched) CD4+ T cells. All TPs have been pooled.
(D) Median frequency and 25th and 75th percentiles of CXCR3+ CCR6+ (Th1 Th17-enriched) CD4+ T cells at each TP.
(E) Representative plot showing ACE2 and isotype staining within the T cell compartment of PMA and ionomycin-restimulated (5 h) COVID-19 samples.
(F) Median frequency and 25th and 75th percentiles of ACE2-positive cells in FlowSOM-generated immune cell clusters after PMA and ionomycin restimulation (5 h). All TPs have been pooled.
(G) Median expression of various markers in FlowSOM-derived clusters of PMA and ionomycin-restimulated (5 h) samples.
(H) Median expression and 25th and 75th percentiles of PD-1 (left panel) and CTLA-4 (right panel) in FlowSOM-generated immune cell clusters after PMA and ionomycin restimulation (5 h). All TPs have been pooled.
∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001, Mann-Whitney test, BH correction. See also Figure S7.