Table 2. Nucleotide composition of mitochondrial genomes of the six Chironomidae species.

	Species	Whole genome	Protein coding genes	First codon position	Second codon position	Third codon position	tRNA genes	12S rRNA	16S rRNA	Control region
A+T%	Chironomus tepperi	76.7	74.3	67.6	67.6	87.6	79.0	82.6	84.3	93.0
	Potthastia sp.	76.8	74.7	69.0	66.1	88.9	76.8	78.1	82.7	93.3
	Rheocricotopus villiculus	77.3	74.4	69.6	67.4	86.0	79.5	84.1	84.4	93.7
	Parochlus steinenii	72.4	69.0	64.6	64.7	77.5	73.2	76.4	80.1	85.5
	Prodiamesa olivacea	75.8	73.4	66.7	65.5	88.2	76.2	78.1	81.9	89.2
	Clinotanypus yani	75.0	72.5	65.4	65.1	87.0	75.7	79.1	81.3	88.7
AT-Skew	Chironomus tepperi	−0.14	−0.20	−0.09	−0.41	−0.13	0.03	−0.01	0.00	−0.11
	Potthastia sp.	−0.13	−0.20	−0.10	−0.39	−0.12	0.03	0.01	0.04	−0.05
	Rheocricotopus villiculus	−0.12	−0.18	−0.09	−0.40	−0.08	0.02	0.01	0.05	−0.07
	Parochlus steinenii	−0.11	−0.19	−0.09	−0.40	−0.11	0.04	0.05	0.03	0.06
	Prodiamesa olivacea	−0.12	−0.19	−0.10	−0.40	−0.09	0.03	0.04	0.01	0.02
	Clinotanypus yani	−0.13	−0.19	−0.10	−0.39	−0.10	0.02	0.00	0.03	−0.08
GC-Skew	Chironomus tepperi	−0.06	−0.02	0.19	−0.18	−0.12	−0.13	−0.37	−0.36	−0.43
	Potthastia sp.	−0.03	0.02	0.27	−0.16	−0.15	−0.12	−0.24	−0.28	−0.31
	Rheocricotopus villiculus	−0.04	−0.01	0.25	−0.18	−0.17	−0.09	−0.23	−0.34	−0.19
	Parochlus steinenii	−0.04	−0.01	0.21	−0.17	−0.10	−0.06	−0.21	−0.26	−0.18
	Prodiamesa olivacea	−0.04	0.00	0.25	−0.16	−0.21	−0.09	−0.24	−0.29	−0.16
	Clinotanypus yani	−0.06	0.00	0.24	−0.18	−0.18	−0.12	−0.28	−0.34	−0.39