Figure 2.

In vitro analysis of soluble markers released by human coronary artery endothelial cells and blood elements in contact with CD31-mimetic Cobalt Chromium discs. (A and B) Quantitative analysis of coagulation and inflammation soluble factors released in the supernatant of human coronary artery endothelial cells cultured during 48 h (A) or released in the blood-derived plasma after 1 h of incubation under continuous rotation (B), in the presence of CD31-mimetic Cobalt Chromium-coated discs or their controls. Individual points are median values from eight replicates of four different donors. The release of plasminogen activator-1, interleukin-6, interleukin-8, E-selectin in the supernatant of human coronary artery endothelial cells and the secretion of tissue factor, plasminogen activator-1, tissue factor pathway inhibitor, CD40L, macrophage inflammatory protein-1 alpha in the plasma derived from the blood incubated with the experimental discs was significantly reduced by the presence of the CD31 peptide, regardless of the type of linker arm used [i.e. poly(ethylene glycol) bis(carboxymethyl) ether or polydopamine]. Furthermore, human coronary artery endothelial cell release of soluble tissue factor pathway inhibitor was significantly increased in the presence of the CD31-mimetic peptide compared to bare and poly(ethylene glycol) bis(carboxymethyl) ether controls, reflecting a CD31-dependent physiologic anticoagulant function of the endothelium. ICAM-1, intercellular adhesion molecule-1; PDGF-BB, platelet-derived growth factor-BB; VCAM-1, vascular cell adhesion molecule 1. Circles: plasma amination; squares: dip-coating.