FIGURE 3.

Overexpression of CircPVT1 promoted differentiation, proliferation and inhibited apoptosis of BMSCs. After 1600 mg of GC, CircPVT1 overexpression plasmid was also given to treat BMSCs. A,B, The expression of CircPVT1 and miR‐21‐5p were detected by RT‐PCR. C, RT‐PCR was taken to assess the levels of osteogenic differentiation markers ALP, RUNX2, CoL1a1, OCN and the expression of osteoclast‐related genes ACP5 and CTSK. D,E, BrdU and flow cytometry were conducted to examine cell proliferation and apoptosis ability. F, Western blot detection of Smad7, Smad2/3, Bax and Bcl2 protein expression. *P < .05, **P < .01, ***P < .001 (vs.con group). ##P < .01, ###P < .001 (vs.GC group). N = 3