FIGURE 6.

Up‐regulation of CircPVT1 inhibited miR‐21‐5p‐induced osteonecrosis of BMSCs. BMSCs were transfected with miR‐21‐5p mimics and/or CircPVT1 overexpression plasmids. A‐F, RT‐PCR was employed to assess the levels of osteogenic differentiation markers ALP, RUNX2, CoL1a1, OCN and the expression of osteoclast‐related genes ACP5 and CTSK. G‐H, BrdU and flow cytometry were used to detect BMSC proliferation and apoptosis. I, Western blot detection of Smad7, Smad2/3, Bax, Bcl2 protein expression. **P < .01, ***P < .001 (vs.con group). ##P < .01, ###P < .001 (vs.miR‐21‐5p group). N = 3