FIGURE 7.

The effect of overexpressed CircPVT1 on osteogenic differentiation of rat femoral head necrosis was verified in vivo. The CircPVT1 overexpression plasmid was injected into the ventricle of SIONFH rats. A, HE staining was taken to check the pathological damage of the femur. B, TRAP staining detected osteoclast viability. C‐E, Measurement of bone cavity rate, cavity area and trabecular area. F‐J, Determination of bone volume fraction, trabecular bone quantity, trabecular bone thickness, trabecular bone separation and mode factor. K‐P, RT‐PCR was used to detect osteogenic differentiation‐related genes ALP, RUNX2, CoL1a1, OCN and osteoclastogenesis‐related genes ACP5 and CTSK. NS P > .05, **P < .01, ***P < .001 (vs.vector group). N = 5