FIGURE 3.

MiR‐93 exhibited the greatest different expression in macrophage‐derived exosomes, contributing to the inhibition of pyroptosis in TCMK‐1 cells. A, Volcano plot showing the distribution of differentially expressed miRNAs in M1 and M2 exosomes. B, qRT‐PCR was used to verify the miRNAs level in M1 and M2 exosomes. C, D, TCMK‐1 cells were treated with miRNA mimic and the indicated control, and then, ELISA was used to measure the IL‐1β and IL‐18 level in the culture medium. E, NLRP3 expression in TCMK‐1 cells was detected by Western blot. F, TCMK‐1 cells were overexpressed with miR‐93‐5p, or treated with M1 and M2 exosomes, and IL‐1β and IL‐18 level in the culture medium was measured by ELISA. G, Cell viability of TCMK‐1 cells in the indicated group was measured by MTT. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001, compared to the indicated group