FIGURE 6.

miR‐18b down‐regulates Notch2 expression, thus promoting the proliferation and migration of trophoblasts in PE‐EV^agomir‐NC, PE‐EV^{miR‐18b agomir}, PE‐EV ^{antagomir‐NC} or PE‐EV^{miR‐18b antagomir}, PE‐EV^miR‐18b + Notch2, PE‐EV^miR‐18b + Vector groups. A, The expression of Notch2 after co‐culture determined using immunoblotting. B, The migration of trophoblasts determined by Transwell assay (×200). C, The cell cycle of trophoblasts determined by flow cytometry. D, The proliferation of trophoblasts determined by CCK‐8 assay. *P < .05 vs the PE‐EV^agomir‐NC group; #P < .05 vs the PE‐EV^{antagomir‐NC} group; & P < .05 vs the PE‐EV^miR‐18b + Vector group. Comparisons among multiple groups were conducted using one‐way ANOVA, followed by Turkey's post hoc test. Variables were analysed at different time points using Bonferroni‐corrected repeated measures ANOVA