. 2021 Apr 9;25(10):4583–4595. doi: 10.1111/jcmm.16234

TABLE 1.

Primer sequences for RT‐qPCR

Target gene	Primer sequences
miR‐18b	F: 5′‐GAAAGAAGGCGAGGAGCAGATCGAGGAA‐3′
miR‐18b	R: 5′‐GCGGTAAGGTGCATCTAGTG‐3′
Notch2	F: 5′‐CACAGGAGAGGACTGCCAATACT‐3′
Notch2	R: 5′‐TGTCCCGGCTGAGCATGT‐3′
TIM3	F: 5′‐TGCTGCTGCTGCTACTACTTACA‐3′
TIM3	R: 5′‐AGGTTGGCCAAAGAGATGAG‐3′
Rheb1	F: 5′‐GCCCAGAACATCTGTTCCAT‐3′
Rheb1	R: 5′‐GGTACCCACAACCTGACACC‐3′
Rhebl1	F: 5′‐GGAAAGAAGCTGGCAGAGTCCTG‐3′
Rhebl1	R: 5′‐CTAGAGGCCAGTGTCCATGAGAG‐3′
β‐actin	F: 5′‐AGTGTGACGTGGACATCCGCAAAG‐3′
β‐actin	R: 5′‐ATCCACATCTGCTGGAAGGTGGAC‐3′
U6	F: 5′‐GCTTCGGCAGCACATATACTAAAAT‐3′
U6	R: 5′‐CGCTTCACGAATTTGAGTGTCAT‐3′

Abbreviations: F, Forward; miR‐18b, MicroRNA‐18b; Notch2, Notch receptor 2; R, Reverse; RT‐qPCR, reverse transcription quantitative polymerase chain reaction; TIM3, T cell immunoglobulin and mucin domain‐containing protein 3.