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. 2021 Apr 26;8:609300. doi: 10.3389/fcvm.2021.609300

Figure 2.

Figure 2

Effect of Ang II on atrial fibrosis. The morphology of rat atrial fibroblasts was observed under an inverted microscope (A). Immunofluorescence fluorescence of Vimentin was utilized to identify atrial fibroblasts (B). CCK-8 assay (C) and BrdU assay (D) were adopted for measurement of the effect of Ang II on the proliferation ability of rat atrial fibroblasts, TUNEL staining for assessment of the apoptosis rate of atrial fibroblasts (E) and cell scratch assay for the performance of migration ability of rat atrial fibroblasts (F). The effect of Ang II on the expression of miR-205 was determined by qRT-PCR (G). The expression of collagen I, α-SMA, and P4HA3 in rat atrial fibroblasts were inspected by qRT-PCR (H) and Western blot (I); *P < 0.05, **P < 0.01; Ang II, angiotensin II.