Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 26;8:609300. doi: 10.3389/fcvm.2021.609300

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Xiao, Reddy, Xue, Liu, Chen, Li, Ling and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

P4HA3 is a target gene of miR-205 in rat atrial fibroblasts. After transfection with miR-205 mimic or miR-205 inhibitor, the mRNA expression of miR-205 was detected by qRT-PCR (A), and the mRNA and protein levels of P4HA3 were measured by qRT-PCR (B) and Western blot (C). RIP experiments verified that Ago2 antibody significantly enriched the mRNA of miR-205 and P4HA3 (D,E). The binding site of miR-205 and P4HA3 was predicted by TargetScan, and sequences were designed according to the binding site (F). Dual-luciferase reporter gene assay was applied to verify the binding site of miR-205 and P4HA3 (G); *P < 0.05, **P < 0.01, ***P < 0.001; RIP, RNA immunoprecipitation.