Figure 5.

MiR-205 restrains atrial fibrosis through P4HA3 to block JNK pathway. After Ang II injection in rats, the p-JNK expression in atrial tissues was measured by Western blot (A). Western blot was applied to detect the p-JNK expression in Ang II-treated atrial fibroblasts (B). Then, Western blot analysis of p-JNK expression were performed on rat atrial fibroblasts which were transfected with miR-205 mimic, pcDNA3.1-P4HA3, or cotransfected with miR-205 mimic and pcDNA3.1-P4HA3 prior to treatment with Ang II (1 μM) (C); *P < 0.05, **P < 0.01; Ang II, angiotensin II.