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. 2021 Apr 26;12:594376. doi: 10.3389/fimmu.2021.594376

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Copyright © 2021 Grassi, Vanini, De Santis, Romagnoli, Aiello, Casetti, Cimini, Bordoni, Notari, Cuzzi, Mosti, Gualano, Palmieri, Fraziano, Goletti, Agrati and Sacchi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PMN-MDSC frequency correlates with the CD14++CD16- monocyte proportion. (A) Gating strategy used to identify monocyte subsets: in the morphological gate (FSC/SSC) we excluded debris gate (A), and morphologically identified monocytes gate (B). In gate B, we selected CD14++CD16, CD14++CD16+, and CD14-CD16++ monocytes. The percentage of monocyte subsets was calculated in the gate (A) Correlation between PMN-MDSC frequency and CD14++CD16 (B), CD14++CD16+ (C), and CD14-CD16++ (D) monocytes from active TB and LTBI subjects. The Spearman test was applied to evaluate correlations. The p <0.05 was considered statistically significant.