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. 2021 Apr 26;12:594376. doi: 10.3389/fimmu.2021.594376

Figure 5.

Figure 5

PMN-MDSC induce BCG and not MTB growth inhibition into monocytes. Relative Luminescence Unit (A) and Replication index (B) of BCGlux 3 days post infection into purified monocytes alone (Mo) or cultured with not infected purified PMN-MDSC (ratio 1/1, Mo+MDSC), or with not infected monocytes as control (ratio 1/1, Mo+Mo). PMN-MDSC infected with BCGlux alone was also evaluate (MDSC). Results are shown as mean ± SE of four independent experiments. (C) Replication index of BCGlux after 3 days of infection of purified monocytes (Mo) cultured with not infected purified PMN-MDSC (ratio 1/1, Mo+MDSC), or with not infected monocytes as control (ratio 1/1, Mo+Mo) in the presence or absence of polyethylene glycol-Catalase (Cat). Results are show as mean ± SE of replicates of one experiment (cells from one donor). (D) Colony Forming Unit (CFU) of wild type BCG after 3 days of infection of purified monocytes (Mo) cultured with not infected purified PMN-MDSC (ratio 1/1, Mo+MDSC), or with not infected monocytes as control (ratio 1/1, Mo+Mo) in the presence or absence of polyethylene glycol-Catalase (Cat). Results are show as mean ± SE of replicates of one out of two independent experiments (cells from two donors). A paired T test was used, and p <0.05 was considered statistically significant. Mo, monocytes; PMN-MDSC, polymorphonuclear-myeloid-derived suppressor cells. (E) Colony Forming Unit (CFU) of MTB (H37Rv) after 3 days of infection of purified monocytes (Mo) cultured with not infected purified PMN-MDSC (ratio 1/1, Mo+MDSC), or with not infected monocytes as control (ratio 1/1, Mo+Mo) in the presence or absence of polyethylene glycol-Catalase (Cat). Results are show as mean ± SE of three independent experiments.