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. 2021 Apr 27;45(6):115. doi: 10.3892/or.2021.8066

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — SchB suppresses migration, invasion and EMT of cancer stem-like cells. Cancer stem-like cells (NCI-H460-CSCs and H661-CSCs) were treated with 10, 20 or 40 µmol/l SchB and the control group was treated with PBS. (A) Migration was detected using wound healing assays. Scale bar for NCI-H460-CSC, 50 µm; Scale bar for H661-CSC, 100 µm. (B) Transwell assays were performed to assess cell invasion in cancer stem-like cells. Scale bar for NCI-H460-CSC, 50 µm; Scale bar for H661-CSC, 100 µm. (C) Protein expression levels of EMT markers including E-cadherin, vimentin and N-cadherin were determined by western blot analysis. All experimental data are shown as the mean ± SD (n=3). Differences were analyzed via one-way ANOVA with Tukey's correction. *P<0.05, **P<0.01 vs. control. SchB, Schisandrin B; EMT, epithelial-mesenchymal transition.