Skip to main content
. 2021 Apr;10(4):1841–1856. doi: 10.21037/tlcr-21-299

Figure 4.

Figure 4

BRF2 was a direct target of let-7b-3p. (A) The expression of BRF2 in TCGA database. (B) BRF2 expression in clinical specimens was detected by qRT-PCR (n=50). (C) HE staining of LUAD tumor tissues and adjacent normal tissues and the expression of BRF2 was detected by IHC. (D,E) BRF2 expression in cell lines was assessed by qRT-PCR and WB. (F) Pearson’s correlation analysis between let-7b-3p and BRF2 mRNA expression in LUAD tumor tissues. (G) BRF2 3'UTR luciferase reporter assays in HEK293 cells. (H) The predicted binding site between let-7b-3p and BRF2 3'UTR. (I) BRF2 protein expression in transfected A549 and H1299 cells was assessed by WB (*, P<0.05; **, P<0.01; ****, P<0.0001). BRF2, TFIIB-related factor 2; TCGA, The Cancer Genome Atlas; qRT-PCR, quantitative real-time polymerase chain reaction; HE, hematoxylin and eosin; LUAD, lung adenocarcinoma; IHC, immunohistochemistry; 3'UTR, 3' untranslated region.