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. 2021 Apr;10(4):1841–1856. doi: 10.21037/tlcr-21-299

Figure 5.

Figure 5

BRF2 reversed let-7b-3p-induced inhibition of LUAD cell proliferation. (A) The knockdown efficiency of si-BRF2 in A549 cells and the overexpression efficiency of pcDNA3.1-BRF2 in H1299 cells were measured by q-PCR and WB assays. (B) Upregulation of BRF2 reversed the inhibitory effect of let-7b-3p on cell proliferation, while downregulation of BRF2 enhanced this effect. The cells were stained with crystal violet. (C,D) The effects of inhibiting cell cycle progression and accelerating apoptosis of let-7b-3p was reversed by high expression of BRF2, while knockdown of BRF2 enhanced these effects. (E) BRF2 reversed the expression changes of cell cycle and cell apoptosis-related proteins induced by let-7b-3p. Data are presented as mean ± SD according to Student’s t-test (*, P<0.05; **, P<0.01; ***, P<0.001). BRF2, TFIIB-related factor 2; LUAD, lung adenocarcinoma; q-PCR, quantitative polymerase chain reaction; WB, Western blot.