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. 2021 Apr;10(4):1804–1818. doi: 10.21037/tlcr-20-920

Figure 1.

Figure 1

Circ-10720 regulates VIM and affects cell morphology in NSCLC cell lines. (A) Circ-10720 and CUL2 mRNA expression in the NSCLC cell lines A549, HCC44, H23 and H1299 in comparison to the expression in the BEAS2B normal immortalized lung cell line. (B) Heat map showing the expression of 29 EMT genes (data obtained from Cancer Cell Line Encyclopedia) in the four NSCLC cell lines studied. (C) Circ-10720 and CUL2 mRNA expression in HCC44 and A549 cells transfected with a siRNA against circ-10720 in comparison with control cells. (D) Representative Western blot analysis image of VIM and CDH1 after transfecting with circ-10720 siRNA. (E) Quantification of VIM and CDH1 relative protein levels in three independent replicates after silencing with circ-10720 siRNA. (F) Expression levels of miR-1246 in cells transfected with siRNA circ-10720 in comparison with control siRNA. (G) Immunofluorescence using VIM antibody (green) in control and circ-10720 silenced cells. (H) Bright field images of control or circ-10720 silenced cells. All bar plot data represented are the average of at least three independent replicates and error bars represent SEM. *, P<0.05; **, P<0.01. NSCLC, non-small-cell lung cancer; EMT, epithelial-mesenchymal transition; VIM, Vimentin; CDH1, E-cadherin.