Figure 2.

Circ-10720 regulates migration and invasion in NSCLC cell lines. (A) Images of one of the 3 replicates of the results of the wound healing assay for study of migration at 0, 12 and 24 hours after transfection with circ-10720 siRNA or control siRNA. The percentage included in each picture represents the wound closure percentage from 0 h. (B) Quantification of wound closure rates at different time points: 0, 6, 12 and 24 h. (C) Representative image of the matrigel-based invasion assay for HCC44 and A549 cells transfected with circ-10720 siRNA or control siRNA. (D) Quantitative analysis of invasion by dissolving crystal violet stained cells in 10% acetic acid and colorimetric reading of OD at 560 nm. Data is presented as ratio of control. All data represented in bar plots are the average of at least three independent replicates and error bars represents SEM. *, P<0.05; **, P<0.01. NSCLC, non-small cell lung cancer.