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. 2021 May 10;18:15. doi: 10.1186/s12014-021-09321-1

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Protein assignment of detected and validated SARS-CoV-2 peptides: Circos plot of peptides against SARS-CoV-2 proteins (outermost ring). Of the 639-peptide panel (2nd outermost ring), many peptides could be identified using our validation workflow in clinical and cell culture datasets (3rd outermost ring). Peptides derived from ORF9b, papain-like protease, Nsp4, Nsp10, uridylate endoribonuclease (Nsp15) and certain spike protein peptides were only found in cell culture datasets (2nd innermost ring). Final peptides chosen for targeted analysis are annotated in the innermost ring. Circos plot was generated in Galaxy [65]