Figure 7.

(A) Histogram of migration time for ssDNAs at an effective column length of 30 μm through a 100 nm × 100 nm NIM-PMMA nanochannel at a field strength of 280 V/cm. Histograms were fit to a Gaussian and each histogram represents 100 events. (B) Comparison of peak resolution at an effective column length of 60 μm and 30 μm. (C) Calculated theoretical plates for ssDNAs at effective column lengths of 30 μm and 60 μm. N = 5.54 (t_m/w_0.5)² was used to calculate the number of theoretical plates and $\frac{N\times 100}{\mathit{\text{column length }}(\mathit{\text{cm}})}$ to obtained theoretical plates in units of m⁻¹.