Human embryonic expression of PNPLA6. PNPLA6 in situ hybridisation of human embryos as Carnegie stage 19 (E) and stage 23 (A–D and F). (A and D) Horizontal sections with expression in the retina and periventricular zones of the lateral and third ventricles. (B) Eye expression observed in the retina, fovea (arrow), retinal pigment epithelium (arrowhead), choroid, external epithelium and lens (L). (C) Anterior and posterior pituitary expression in the epithelium and parenchyma. (E) PNPLA6 expression in the developing cerebellum (arrow) and hindbrain (arrowhead). (F) PNPLA6 is expressed in the nasal epithelium (arrow), eyelid epithelium (arrowhead), extraocular muscles (EOM) and lens (L). Scale bar represents 400 μm in (A), (D) and (E) 100 μm in (B) and (C), and 200 μm in (F).