Suppression of pnpla6 expression produces developmental defects in zebrafish embryos. (A) Normal embryos class. (B–D) Embryos injected with morpholino (MO) against pnpla6 have developmental defects, curved head (mild), curved head and distal tail (intermediate), and curved, small head with full tail curvature (severe). (E) Percentage of embryos categorised in various phenotypic classes after injection with pnpla6 MO, wild-type mRNA or coinjection of PNPLA6 harbouring mutant alleles (N>100 embyros per condition; error bars=SEM; *p<0.05, **p<0.01, ***p<0.001).