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. Author manuscript; available in PMC: 2021 May 10.
Published in final edited form as: J Invest Dermatol. 2017 Sep 28;138(3):500–510. doi: 10.1016/j.jid.2017.09.024

Figure 4. Delayed hair follicle morphogenesis in AZIPtg/+ lipodystrophic mice.

Figure 4.

Analysis of back skin sections from AZIPtg/+ and WT mice. (a) Hematoxylin and eosin staining. Scale bar = 100 μm. (b) Quantitative histomorphometry at P1. Hair morphogenesis score (left). Percentage of HFs found in the different hair morphogenesis stages (right panel; n = 9–12). (c) Gene expression of K6irs, Tchh, mHa2 and mHa3, and Foxn1 in skin from AZIPtg/+ and WT mice (P5). Data are normalized to Eef1α1 and expressed as mean ± standard error of the mean (n = 7). (d) Oil Red O staining and CD45 immunostaining (leucocytes, in red) and (e) CD45 quantification. Scale bar = 200 μm. *P < 0.05, **P <0.01 and ***P < 0.001, with respect to control at the same time point. H&E, hematoxylin and eosin; HM, hair morphogenesis; P, postnatal day; WT, wild type.