The correlation effect between P2X3 and TRPV1 of CFA rats in vivo. (a) After TRPV1 was activated, the flinching times (2 min intervals) of CFA rats with P2X3 agoinst/P2X3 antagonist. (b) After TRPV1 was activated, the flinching times (10 min intervals) of CFA rats with P2X3 agoinst/P2X3 antagonist. (c) After TRPV1 was activated, the licking time (2 min intervals) of CFA rats with P2X3 agoinst/P2X3 antagonist. (d) After TRPV1 was activated, the licking time (10 min intervals) of CFA rats with P2X3 agoinst/P2X3 antagonist. Compared with CFA+vehicle+capsaicin group, △P<0.05, △△P<0.01, compared with CFA+α,β-meATP+capsaicin group, #P<0.05, ##P<0.01, (CFA+vehicle+capsaicin group: n=4, CFA+α,β-meATP+capsaicin group: n=5, CFA+TNP-ATP+capsaicin group: n=5). (e) After P2X3 was activated, the flinching times (2 min intervals) of CFA rats with TRPV1 agoinst/TRPV1 antagonist. (f) After P2X3 was activated, the flinching times (10 min intervals) of CFA rats with TRPV1 agoinst/TRPV1 antagonist. (g) After P2X3 was activated, the licking time (2 min intervals) of CFA rats with TRPV1 agoinst/TRPV1 antagonist. (h) After P2X3 was activated, the licking time (10 min intervals) of CFA rats with TRPV1 agoinst/TRPV1 antagonist. Compared with CFA+vehicle+α,β-meATP group,△P<0.05, △△P<0.01, compared with CFA+capsazepine+α,β-meATP group, #P<0.05, ##P<0.01, (CFA+vehicle+α,β-meATP group: n=5, CFA+capsaicin+α,β-meATP group: n=5, CFA+ capsazepine +α,β-meATP group: n=6).