Figure 2.

FKBP5 was effectively depleted in TPC-1 and KTC-1 thyroid cancer cell lines. (a) Quantitative polymerase chain reaction detection of FKBP5 expression in TPC-1 and KTC-1 cells transfected with control or FKBP5 shRNA plasmids. (b) Western blot detection of FKBP5 expression in TPC-1 and KTC-1 cells transfected with control or FKBP5 shRNA plasmids (upper). Expression levels of β-actin and FKBP5 were evaluated using ImageJ with integrated optical density based on the results of immunoblotting. Relative FKBP5 expression was normalized to β-actin level. *P<0.05

shRNA, short hairpin RNA.