Figure 4.

FKBP5 suppressed the apoptosis of TPC-1 and KTC-1 thyroid cancer cells. (a) Apoptosis of TPC-1 and KTC-1 cells transfected with FKBP5 shRNA plasmids was detected by colony-formation assays. (b) Immunoblot detection of caspase-3 protein expression after FKBP5 ablation (upper). Expression levels of β-actin and caspase-3 were evaluated using ImageJ with integrated optical density (IOD) based on the results of immunoblotting. Relative caspase-3 expression was normalized to β-actin level. (c) Immunoblotting of caspase-7 protein expression after FKBP5 knockdown (upper). Expression levels of β-actin and caspase-7 were evaluated using ImageJ with IOD based on the results of western blotting. Relative caspase-7 expression was normalized to β-actin level. (d) Immunoblotting of caspase-9 protein expression after FKBP5 knockdown (upper). *P < 0.05

shRNA, short hairpin RNA.