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. 2020 Oct 7;70(6):1010–1013. doi: 10.1136/gutjnl-2020-322945

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© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Fluorescently labelled peptides for multiplexed imaging. Biochemical structures are shown for (A) QRH*-Cy5 and (B) KSP*-IRDye800. (C) Peak absorbance of QRH*-Cy5 and KSP*-IRDye800 occurs at abs=648 and 776 nm, respectively. Peak fluorescence emits at em=675 and 812 nm, respectively. (D) Schematic diagram for the multimodal scanning fibre endoscope (mmSFE) is shown. Excitation at ex=638 and 785 nm is delivered through a single-mode fibre (SMF) that is scanned in a spiral pattern by a piezo tube actuator. The beam is focused onto the tissue surface (illumination plane) by a lens assembly. (E) Fluorescence is collected by a ring of large core multi-mode fibres (MMF) mounted around the instrument periphery. (F) The dimensions of the rigid tip are 9 mm in length and 2.4 mm in diameter. (G) This instrument passes forward through the 2.7 mm working channel of a standard medical endoscope (Olympus #GIF-HQ190). (H) The system is contained within a portable cart.