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. 2020 Oct 9;70(6):1023–1036. doi: 10.1136/gutjnl-2020-321731

Figure 3.

Figure 3

The monocyte response to PRR stimulation is heterogeneous, revealing a paracrine mechanism of IL-10-depedent regulation of IL-23 production. (A) Representative contour plot presentation showing IL-23p19+ and IL-10+ frequencies in monocytes derived from a healthy donor and a patient with IBD at 16 hours poststimulation. (B) IL-23p19+IL-10–, IL-23p19+IL-10+ and IL-23p19–IL-10+ monocyte frequencies at 16 hours poststimulation (HD: n=26, IBD: n=18; mean±SEM; Friedman test with FDR-adjusted p values). (C) Monocyte cytokine frequencies according to (A) and (B), comparing patient subgroups and stimulation conditions (HD: n=26, IBD: n=18; mean±SEM, Mann-Whitney test). (D) Representative dual-colour ELISpot images showing non-stimulated, LPS-stimulated and combined LPS and anti-IL-10R-stimulated monocyte IL-10 secretion (blue), IL-12p40+IL-23p19+ secretion (red) and combined production of both cytokines (violet/brown). Numbered magnifications of distinct spots detected in the LPS+anti-IL-10R-stimulated condition are shown on the right. (E) Dual-colour ELISpot counts from three independent experiments (n=6, Mann-Whitney test). Ctrl, control; FDR, false discovery rate; IL, interleukin; n.s., not significant; LPS, lipopolysaccharide; SFU, spot forming units. *pvalue<0.05, **pvalue<0.01, ***pvalue<0.001, ****pvalue<0.0001.