Fig. 5.

Biglycan deletion enhances drug delivery and antitumor effect of paclitaxel in breast cancer. a Representative images of CD31 immunostaining (green), doxorubicin (red), and DAPI nuclear staining (blue) in E0771 tumors from WT and Bgn KO mice. Scale bar = 100 μm. b Quantification of drug delivery. Significance was determined by two-tailed Student’s t test. n = 5 fields per mouse, 5 mice per group. c Growth curves of E0771 tumors grown in WT and Bgn KO mice. Mice were treated with DMSO or 2 mg/kg paclitaxel once every 3 days for a total of 6 treatments. Significance was determined by a one-way ANOVA. n = 4–5 mice per group. d, e Representative IVIS images of d metastatic lymph nodes and e lungs in E0771 tumors of DMSO- or paclitaxel-treated WT and Bgn KO mice as described in c. All data represent means ± SD. *p < 0.05, ***p < 0.001