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. 2021 May 10;22:129. doi: 10.1186/s13059-021-02340-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — HDAC4 is required for senescence escape. a Immunoblot analysis in BJ/hTERT/E1A/RAS/HDAC4^+/+ or ^−/− cells, as indicated. Actin was used as loading control. b Analysis of the senescent cells as scored after SA-β-gal staining. Mean ± SD; n = 3. c RNA-expression levels of the indicated genes in BJ/hTERT/E1A/RAS/HDAC4^−/− with respect to WT cells. Mean ± SD; n = 3. d Immunoblot analysis in BJ/hTERT cells expressing the indicated transgenes. Where indicated, HRAS^G12V was induced for 9 days. Anti-GFP was used to visualize GFP-NFKBIA. Actin was used as loading control. e Analysis of the senescence as scored after SA-β-gal staining in BJ/hTERT cells expressing the indicated transgenes. f Analysis of the population doublings in BJ/hTERT cells expressing the indicated transgenes, scored during the 9 days of observation. g RNA-expression levels of the indicated genes in the cells co-expressing RAS and the indicated transgenes, with respect to HYGRO expressing cells not expressing RAS. Mean ± SD; n = 3. The significance is relative to RAS+Hygro^R co-expressing cells