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. 2021 May 10;22:129. doi: 10.1186/s13059-021-02340-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — The effect of a forced localization of HDAC3 or HDAC4 on the epigenetic status of selected SES. a Schematic representation of the experimental strategy. b ChIP-qPCR signals using the anti-Cas9 antibody for the indicated loci in BJ/hTERT/RAS cells transfected with the indicated dCas9 chimeras and IVT sgRNAs. The genomic region investigated is indicated with the closest-associated gene. c ChIP-qPCR signals using the anti-Cas9 antibody for the indicated loci in BJ/hTERT/RAS cells transfected with the indicated dCas9 chimeras and IVT sgRNAs. The genomic region is indicated by the relative coordinates. d ChIP-qPCR signals using the anti-H3K27ac antibody for the indicated loci in BJ/hTERT/RAS cells transfected with the indicated dCas9 chimeras and IVT sgRNAs. The genomic region investigated is indicated with the closest-associated gene. e ChIP-qPCR signals using the anti-H3K27ac antibody for the indicated loci in BJ/hTERT/RAS cells transfected with the indicated dCas9 chimeras and IVT sgRNAs. The genomic region is indicated by the relative coordinates. The sgRNAs used are indicated for each bar (e.g., dCas9 IL1B/CXCL8 means that 4sgRNA for IL1B and 4 for CXCL8 were used). Data are reported as mean ± SD and normalized on total H3 signal