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Comparison of E and N1 Singleplex and Duplex E/RNase P assays.
(A) Clinical samples (n = 196) were assayed using the E primers and FAM-labelled probe alone (E singleplex) or in combination with RNase P primers and HEX-labelled probe (E/RNase P Duplex) (B) Clinical samples (n = 422) were assayed using the CDC N1 primers and FAM-labelled probe alone (N1 singleplex) or with a combination with E and RNase P primers and probes (E/RNase P Duplex).
