Table 1.

Primers and probes used in this study.

	Sequence (5′ to 3′)	Working concentration
E_Sarbeco_F (Corman et al., 2020)	5′-ACAGGTACGTTAATAGTTAATAGCGT-3′	400 nM
E_Sarbeco_R (Corman et al., 2020)	5′-ATATTGCAGCAGTACGCACACA-3′	400 nM
E_Sarbeco_P1 (Corman et al., 2020)	5′-FAM-ACACTAGCCATCCTTACTGCGCTTCG-BBQ-3′	200 nM
RNaseP_F (Centre for Disease Control and Prevention, 2020)	5′-AGATTTGGACCTGCGAGCG-3′	100 nM
RNaseP_R (Centre for Disease Control and Prevention, 2020)	5′-GAGCGGCTGTCTCCACAAGT-3′	100 nM
RNaseP_Pr (Centre for Disease Control and Prevention, 2020)	5′-HEX-TTCTGACCTGAAGGCTCTGCGCG-BBQ-3′	100 nM
2019-nCoV_N1-F (Centre for Disease Control and Prevention, 2020)	5′-GACCCCAAAATCAGCGAAAT-3′	400 nM
2019-nCoV_N1-R (Centre for Disease Control and Prevention, 2020)	5′-TCTGGTTACTGCCAGTTGAATCTG-3′	400 nM
2019-nCoV_N1-P (Centre for Disease Control and Prevention, 2020)	5′-FAM-ACCCCGCATTACGTTTGGTGGACC-BBQ-3′	100 nM