FIGURE 1:

Establishment of Tric-KO, Ocln-KO, and Tric/Ocln-dKO cells. (A, B) Ctrl, Tric-KO #1, and Tric/Ocln-dKO #1 cells (A) and Ctrl, Ocln-KO #1, and Tric/Ocln-dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit anti-ZO-1 pAb (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric-KO and Tric/Ocln-dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric-KO, Ocln-KO, and Tric/Ocln-dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.