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. 2021 Apr 15;32(8):664–674. doi: 10.1091/mbc.E20-11-0748

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FIGURE 3: — Δema19 cells show morphological changes on growth on glycerol. (A, B) Yeast cells were grown to log phase in media containing glucose or glycerol as carbon source. Cells were fixed in 2% glutaraldehyde, 3% formaldehyde overnight at 4°C and treated as described (Prescianotto-Baschong and Riezman, 2002). Sections were analyzed by transmission electron microscope. Arrows point at the morphological structures observed in Δema19 cells. More examples and enlarged images are shown in Supplemental Figure S4. (C, D) The content of lipids in whole cell or mitochondrial extracts was analyzed by mass spectrometry and is shown here as the percentage of total lipid mass. CDP-DAG, cytidine diphosphate diacylglycerol; CL, cardiolipin; PA, phosphatidic acid, PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; PS, phosphatidylserine.