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. 2021 Apr 15;32(8):703–711. doi: 10.1091/mbc.E20-08-0508

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© 2021 Gerganova et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 2: — Ptc1 localization to the plasma membrane is dependent on Skb5 and Mod5. (A) Cell lengths at division quantified from at least three independent experiments; statistical significance measured by Student’s t test across experiments against either wild-type or uninduced counterpart. The indicated variation is the SD across cells. (B) Coimmunoprecipitation of Ptc1-GFP and Pom1-HA. Protein extracts were made from cells grown in 2% glucose for 24 h and from cells shifted to 0.03% glucose for 2 h. Whole cell extract (2 mg) was used for immunoprecipitation assay. Inputs (100 µg) were loaded. Note that the levels of Pom1-HA detected in the inputs are variable across experiments. (C) Pom1 binds Ptc1 in vitro. Bacterially expressed proteins assayed by affinity columns. GST tagged Pom1^FL and fragments Pom1^Δaa305N and Pom1^aa605-1087 tested for interaction with recombinant MBP-tagged Ptc1^FL. Left, MBP-bound proteins were immobilized on amylose columns. Right, GST-bound proteins were bound to glutathione beads.