FIGURE 1:

In mouse cells, ciliary gating is disturbed by the loss of Rpgrip1l but not by the loss of Nphp4. (A) Immunofluorescence on MEFs obtained from WT (n = 5) and Rpgrip1l^–/– (n = 5) embryos. At least 10 cilia per embryo were used for quantifications (Σ(WT) = 62 cilia, Σ(Rpgrip1l^–/–) = 72 cilia). (B) Immunofluorescence on MEFs obtained from WT (Arl13b: n = 4; Sstr3: n = 3) and Nphp4^–/– (Arl13b: n = 4; Sstr3: n = 3) embryos. At least 20 cilia per embryo were used for quantifications (Arl13b: Σ(WT) = 83 cilia, Σ(Nphp4^–/–) = 171 cilia; Sstr3: Σ(WT) = 134 cilia, Σ(Nphp4^–/–) = 92 cilia). (A, B) The ciliary axoneme is stained in green by acetylated α-tubulin, the BB is stained in blue by γ-tubulin. The scale bars represent a length of 0.5 µm. Data are shown as mean ± SEM. Asterisks denote statistical significance according unpaired t tests with Welch´s correction (***P < 0.001) (A: t (25) = 6.54, P < 0.0001; B: Arl13b: t (46) = 0.7483, P < 0.4581; Sstr3: t (80) = 1.807, P < 0.0745).