TABLE 1:
Key resources.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-Actin | Sigma-Aldrich | #A2066 |
| Rabbit polyclonal anti-Arl13b | Proteintech | #17711-1-AP |
| Mouse monoclonal anti-Arl13b | Antibodies Incorporated | #75-287 |
| Rabbit polyclonal anti-Cep290 | Abcam | #ab84870 |
| Rabbit polyclonal anti-Cspp1 | Proteintech | #11931-1-AP |
| Rabbit polyclonal anti-Flag | Sigma-Aldrich | #F7425 |
| Rabbit polyclonal anti-Gapdh | Abcam | #ab9485 |
| Rabbit polyclonal anti-GFP | Thermo Fisher Scientific | #A-6455 |
| Rabbit polyclonal anti-Nphp5/Iqcb1 | Proteintech | #15747-1-AP |
| Rabbit polyclonal anti-c-Myc | Santa Cruz Biotechnology | #sc-789 |
| Rabbit polyclonal anti-Sept2 | Proteintech | #11397-1-AP |
| Rabbit polyclonal anti-Sept7 | Proteintech | #13818-1-AP |
| Rabbit polyclonal anti-Sstr3 | Proteintech | #20696-1-AP |
| Rabbit polyclonal anti-Sstr3 | Pierce Biotechnology | #PA3-207 |
| Goat polyclonal anti-Sstr3 | Santa Cruz Biotechnology |
#sc-11617 |
| Mouse monoclonal anti-acetylated α-Tubulin | Santa Cruz Biotechnology | #sc-23950 |
| Mouse monoclonal anti-acetylated α-Tubulin | Sigma-Aldrich | #T-6739 |
| Goat polyclonal anti-γ-Tubulin | Santa Cruz Biotechnology. | #sc-7396 |
| Goat polyclonal anti-mouse IgG2b Alexa Fluor 488 | Thermo Fisher Scientific | #A21141 |
| Goat polyclonal anti-mouse IgG2a Alexa Fluor 488 | Thermo Fisher Scientific | #A21131 |
| Goat polyclonal anti-mouse IgG1 Alexa Fluor 488 | Thermo Fisher Scientific | #A21121 |
| Goat polyclonal anti-mouse IgG1 Alexa Fluor 568 | Thermo Fisher Scientific | #A21124 |
| Goat polyclonal anti-mouse IgG2b Alexa Fluor 594 | Thermo Fisher Scientific | #21145 |
| Goat polyclonal anti-mouse IgG2a Alexa Fluor 594 | Thermo FisherScientific | #A21135 |
| Donkey polyclonal anti-goat Dylight405 | Jackson ImmunoResearch | #AB_2340426 |
| Donkey polyclonal anti-mouse Alexa488 | Jackson ImmunoResearch | #AB_2340846 |
| Donkey polyclonal anit-rabbit Cy3 | Jackson ImmunoResearch via Dianova | # 111-165-045 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMSO | Sigma-Aldrich | #W387520 |
| Eupatilin | Sigma-Aldrich | #SML1689 |
| Experimental Models: Cell Lines | ||
| HEK293 | DSMZ | #ACC305 |
| NIH3T3 | DSMZ | #ACC59 |
| Oligonucleotides | ||
| Rpgrip1l-T3b-for:GAATGGCCACCAAGTTAATACGGCTAG | This study | N/A |
| Rpgrip1l-T3b-rev:CTTCAGGATCTGACAGAGAGCAAGCCTC | This study | N/A |
| T3 off-1 for:CTGTCAGGTTTCCCAGTGTGCAG | This study | N/A |
| T3 off-1 rev:CTCTCAGCTCCTTTTAGGTCTCCAG | This study | N/A |
| T3 off-2 for:ATCCAGCCAAACCCTGCCTGTTC | This study | N/A |
| T3 off-2 rev:GGTTTGTCTCTGTCCTGACATGTCAC | This study | N/A |
| T3 off-3 for:GTCTCCTTCAGACCCACTGAAGTG | This study | N/A |
| T3 off-3 rev:GTCCCAGGAAGCCAGGCTGTTG | This study | N/A |
| Recombinant DNA | ||
| pMyc-mNphp1 | Kindly provided by Sophie Saunier | N/A |
| pFlag-mCep290 | Addgene | #27381 |
| eGFP-hCEP290 | Kindly provided by Hemant Khanna | N/A |
| Software and Algorithms | ||
| ImageJ | National Institutes of Heath | https://imagej.nih.gov/ij |
| AxioVision Rel. 4.8 software package | Carl Zeiss AG | https://www.zeiss.de/mikroskopie |
| NIS-Elements | Nikon | https://www.microscope.healthcare.nikon.com |
| Adobe Photoshop CS2 | Adobe | https://www.adobe.com |
| GraphPad Prism | GraphPad | https://www.graphpad.com |